RPMI 1640 Medium, 1X Liq. without L-Glutamine, L-Cysteine, L-Cystine and L-Methionine

RPMI 1640 Medium was originally developed by Moore and his colleagues at Roswell Park Memorial Institute (RPMI). It was based on the RPMI 1630 line of media which utillized a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins.

RPMI has successfully been used for the cultivation of normal human and neoplastic leukocytes. It is now a popular general purpose medium when properly supplemented.