Cholesterol Oxidase from Streptomyces species

CAS # : 9028-76-6

Form: Lyophilized powder

Activity: Approximately 15 units/mg solid

Stability: Stable at -20°C. A decrease in activity of approximately 10% may occur at 2 to 8°C within 6 months.

Stabilizers: Bovine serum albumin, sugars.

Isoelectric point: 5.1 ± 0.1 and 5.4 ± 0.1

Michaelis Constant: 4.3 x 10-5 M (Cholesterol)

Inhibitors: Ionic detergents, Hg++, Ag+

Optimum pH: 6.5 to 7.0

Optimum Temperature: 45° to 50°C

pH Stability: 5.0 to 10.0 (25°C, 20 hr)

Thermal Stability: below 45°C (pH 7.0, 15 minutes)

This enzyme is useful for the determination of cholesterol in serum by coupling with cholesterol esterase