Mitomycin C, Thermo Scientific Chemicals

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

General Description

• Mitomycin C is a methylazirinopyrroloindoledione compound isolated from the bacterium Streptomyces caespitosus, and other Streptomyces bacterial species that has antineoplastic and antibiotic activities
• This compound acts as an inhibitor of DNA synthesis, nuclear division, as well as an apoptosis inducer. It can create oxygen radicals, and produce inter-strand DNA crosslinks, inhibiting DNA synthesis
• Mitomycin C shows alkylating activity and mainly targets the guanine nucleoside sequence 5′-CpG-3′. It inhibits DNA synthesis by covalently reacting with DNA, forming crosslinks between complementary strands of DNA. Consequently, this interaction prevents the separation of complementary DNA strands, inhibiting DNA replication

Application

• Mitomycin C is active against a broad-spectrum of gram-positive, gram-negative, and acid-fast bacilli bacteria
• Mitomycin C has strong antineoplastic activity, mainly against Ehrlich ascites tumor cell lines
• This compound can inhibit B and T cells and macrophage proliferation. It can also impair antigen presentation, as well as the secretion of interferon-γ, TNF- α, and IL-2
• This compound is used to generate mitotically inactive feeder cells in cell culture systems, such as the mitotically inactive fibroblasts used in embryonic stem cell systems
• It has been used to treat basal-like cancer cell line in vitro experiments
• It is particularly toxic to hypoxic cells inhibiting RNA and protein synthesis at high concentrations