Search Thermo Fisher Scientific
Fisher Catalogue
HiTrap™ TALON™ Crude Prepacked Columns
For purification of histidine-tagged recombinant proteins by immobilized metal affinity chromatography (IMAC). Cytiva Lifescience™ HiTrap™ TALON™ Crude Prepacked Columns offer a cobalt-based medium allowing protein purification under native or denaturing conditions.
Have Questions?
Change view
| Catalog Number | Quantity |
|---|---|
| FKS11618893 | 5 x 1 mL |
| FKS12034118 | 5 x 5 mL |
Catalog number FKS11618893
View Price:Sign InSign in to see your account pricing. Need an account? Register with us today.
Quantity:
5 x 1 mL
Cobalt-based IMAC medium offering different selectivity compared to nickel-charged media. Allows protein purification under native or denaturing conditions and can be used with prokaryotic and eukaryotic expression systems.
- Prepacked with cobalt-based chromatography media
- Binding capacity approximately 7mg MBP
- Maximum pressure: 3 bar
- Column material: polypropylene
- Compatible with commonly used buffers and easily regenerated with 0.5 M NaOH
- 1 and 5mL columns
Media
- Ligand: TALON™ Superflow Cobalt-IMAC
- Matrix: 6% cross-linked agarose
- Average particle size: 60 to 160μm
- pH stability working range: 3 to 12
1mL columns
- Column I.D.: 7mm
- Flow velocity: 1 to 4mL/min.
5mL columns
- Column I.D.: 16
- Flow velocity: 5 to 20mL/min.
Specifications
Quantity5 x 1 mL
For Use With (Application)For PurifiCatIon of Histidine-Tagged Recombinant Proteins by Immobilized Metal Affinity Chromatography, Used Buffers and Easily Regenerated with 0.5 M NaOH
Storage Requirements4°C to 8°C
Binding CapacityUp to 20 mg Histidine-tagged Protein1
Diameter (English) Inner0.27 in.
Product TypeHiTrap Talon crude prepacked Column
Diameter (Metric) Inner0.7 cm
Flow Rate1 to 4 mL/min
Particle Size60 to 160 μm
MaterialPolypropylene
Max. Pressure3 bar
Matrix6% Cross-Linked Agarose
pH Range3 to 12
Storage Buffer20% Ethanol
Ligand TypeTalon Superflow Cobalt-IMAC
Packaging Quantity5 x 1 mL
Unit SizePK