Plates and Modules with Affinity Binding Surfaces
Plates and Modules with Affinity Binding Surfaces
Actual product may vary
Plates and Modules with Affinity Binding Surfaces
Plates and Modules with Affinity Binding Surfaces
Plates and Modules with Affinity Binding Surfaces

Plates and Modules with Affinity Binding Surfaces

Help reduce variability in molecular orientation with these plates and modules with affinity binding surfaces.
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Catalog NumberColorProduct TypeSurface TreatmentTotal Volume (Metric) Well
436022ClearLock-Well Module C8 (x12) in FrameStreptavidin350 μL
436014ClearMicroplateStreptavidin400 μL
Catalog number 436022
Price (SGD)
1,262.00
CS
Add to cart
Color:
Clear
Product Type:
Lock-Well Module C8 (x12) in Frame
Surface Treatment:
Streptavidin
Total Volume (Metric) Well:
350 μL
Price (SGD)
1,262.00
CS
Add to cart

Help reduce variability in molecular orientation with Thermo Scientific™ Plates and Modules with Affinity Binding Surfaces.

Affinity Capture Surfaces (Passive Streptavidin and Immobilizer Streptavidin, Nickel Chelate and Glutathione, BioBind):

  • Highly specific binding surfaces reduce variability in molecular orientation.
  • Immobilizer surfaces improve signal to noise ratios.
  • Streptavidin biotin interaction can be exploited to immobilize a wide range of biomolecules.
Specifications
FormatStandard
LidWithout Lid
Detection MethodColorimetric
Array8 x 12
PackagingIndividually Wrapped
Binding TypeAffinity
ColorClear
Product TypeLock-Well Module C8 (x12) in Frame
MaterialPolystyrene
Volume (Metric) Well350 μL
No. of Wells96
Volume (Metric) Working100 μL
SterilityNon-sterile
Surface TreatmentStreptavidin
Total Volume (Metric) Well350 μL
Well ShapeRound, Flat Bottom (F-well)
No. per Case15
No. per Pack1
Plate BlockingUnspecified
Quantity15/Cs.
Target MoleculeBiotinylated
Unit SizeCS

Frequently asked questions (FAQs)

For Nunc Immuno plates and modules, what are the advantages of one well geometry type over another? Which types should I use for various applications?

The following list describes the geometries of wells available for Nunc Immuno-plates and modules:
- Flat bottom (F): Allows maximum transmission of light. These plates can be read on a monochromatic reader.
- Round bottom (U): This geometry optimizes washing and coating. These plates can be read using a dual wavelength reader.
- "C" bottom (C): This design of the well is a combination of both flat and round bottoms. Basically, it is a flat bottomed well with curved edges at the bottom. These plates also can be read using a monochromatic reader combining optimal reading and washing.
- StarWell: These wells have a modified "C" shape geometry with eight fins strategically placed at the bottom. This feature increases surface area, allowing more molecules to become immobilized which reduces incubation times.

Why am I getting a high background signal when using Nunc Immobilizer Streptavidin Plates/Strips?

High background signal could be due to inadequate washing. Make sure to pre-wash the Nunc Immobilizer Streptavidin Plates/Strips and increase the number of washing steps and the time between washes. Another possible cause is too high a concentration of the detection components.

How long will my Nunc Immobilizer Streptavidin Plates/Strips remain stable?

Our Nunc Immobilizer Streptavidin Plates/Strips are extremely stable over time, if stored at room temperature (20 degrees C to 25 degrees C). If kept in the unopened foil packaging, this product should have an 18 month shelf life after the date of manufacturing.

How long should I incubate the biotinylated and target molecule with the Nunc Immobilizer Streptavidin surface?

Coupling of the target molecule to the streptavidin proceeds very rapidly. Results show that incubation with gentle agitation for 2 hr at room temperature is typically sufficient to saturate all available active site.

What is the binding capacity for free biotin?

The total binding capacity for free biotin is:
- 20 pmol/well in a 96 well (coating volume 100 µL)
- 10 pmol/well in a 384 well (coating volume 50 µL)

View all Plates and Modules with Affinity Binding Surfaces FAQs