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Nutrient Broth is used in many laboratory procedures as is, or with added indicators, carbohydrates, organic liquids, salts, etc.
This medium is used in accordance with official recommended procedures for the bacteriological analyses of water, milk and dairy products, in foods of important sanitation, tests for sensitivity and resistance and as a base to prepare media supplemented with other nutrients.
It can be used for enumeration, isolation and enrichment as well as providing a high grade base for the preparation of special media.
With the addition of blood, ascites fluid or serum, they can also be used to cultivate streptococci, pneumococci and erysipelas species etc.
Nutrient Broth is a general purpose medium used for cultivating a broad variety of fastidious and non-fastidious microorganisms with non-exacting nutritional requirements.
Peptone and yeast extract provide nitrogenous compounds, vitamin B complex, amino acids and other essential growth nutrients.
Sodium chloride is used to maintain the osmotic balance and glucose is the fermentable carbohydrate source of the media.
Specifications
FormTan Powder
Preparation MethodSterilize at 121°C (15 lbs. of pressure) for 15 minutes.
Product TypeMicrobiology Media
FormulationNutrient Broth is a liquid medium, produced according to the formula from APHA and AOAC. Approximate formula:gelatin Peptone: 5g/L; Beef Extract: 3g/L; Final pH: 6.9 +/- 0.1
For Use With (Application)Growth of fastidious and non-fastidious microorganisms