Guanidine Hydrochloride, Ultra Pure grade, MP Biomedicals

Catalog Number	Quantity
MPB04820540	1 kg

Catalog number MPB04820540

View Price:

Quantity:

Request bulk or custom format

Specifications

Chemical Name or MaterialGUANIDINE HYDROCHLORIDE

CAS50-01-1

Melting Point180°C to 190°C

ColorWhite

Recommended StorageStore at Room Temperature (15-30°C). Store Desiccated.

Guanidine Hydrochloride is a strong chaotropic agent useful for the denaturation and subsequent refolding of proteins
This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies
This can be used as the first step in refolding proteins or enzymes into their active form
Urea and dithiothreitol (DTT) may also be necessary
Guanidine HCl is used in the isolation of RNA to dissociate the nucleoprotein into its nucleic acid and protein moieties
It is an inhibitor of RNase
Highly concentrated (6 - 8 M) Guanidine HCl solutions are used to denature native globular proteins
It apparently disrupts hydrogen bonds which hold the protein in its unique structure; however, there also is evidence suggesting that guanidine hydrocholoride may disrupt hydrophobic interactions by promoting the solubility of hydrophobic residues in aqueous solutions