Tris, MP Biomedicals™

Tris is relatively non-hygroscopic; however, if needed, Tris (base) can be dried at 100°C for up to 4 hours to remove any water. Neither Tris nor Tris Hydrochloride by themselves provide adequate buffering capacity. Generally the two need to be mixed together to provide a buffer with pH between 7 and 9 to provide adequate buffering. The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per°C decrease in temperature, and decreases 0.03–0.05 unit per ten-fold dilution.