Hyaluronidase, Ovine testes

Hyaluronidase catalyzes the random hydrolysis of 1,4-linkages between 2-acetamido-
2-deoxy- b-D-glucose and D-glucose residues in hyaluronate.

• CAS # : 37326-33-3
• Synonyms: Hyaluronate 4-glycanohydrolase; hyaluronate lyase; hyaluronoglucosaminidase; E.C. 3.2.1.35
• Physical Description: Off white crystalline powder
• Unit Definition: That amount of enzyme causing the same turbidity reduction as the International Unit (I.U.) as compared with the international standard.
• Molecular weight: Borders and Raftery (1968) reported a molecular weight of 61,000. Khorlin, et. al.(1973) report four subunits of 14,000 each and a total molecular weight of 55,000.
• Solubility: Dissolves readily at 5mg/mL in 0.02M sodium phosphate/0.45% sodium chloride/NaOH/0.01% BSA pH 6.9 to give a clear colorless solution .
• Optimum pH: 4.5 - 6 (DeSalequi et al. 1967).
• Extinction coefficient: = approximately 8.
• Inhibitors: Fe2+ and Fe3+ are inhibitory as are Mn2+ and Cu2+(Warren et al. 1962).
• Specificity: Testicular hyaluronidase hydrolyzes the endo-N-acetylhexosaminic bonds of hyaluronic acid and chondroitin sulfuric acids A and C (but not B), primarily to tetrasaccharide residues (Ludowieg et al. 1961). Monosaccharides are not liberated (Rappaport et al. 1951).
• Stabilizers: Yang and Srivastav (1975) report that sodium chloride acts as a stabilizer .