PureLink™ HiPure Plasmid Filter Midiprep Kit

PureLink HiPure Plasmid Filter Midiprep Kit is designed to isolate plasmid DNA of highest purity from 25 to 100 mL of overnight bacterial culture. The PureLink HiPure Plasmid Filter Kits include HiPure Filter Columns, which combine filtration for clarification of bacterial lysate with anion-exchange resin purification of plasmid DNA, in single unit. The resulting plasmid DNA is of quality equivalent to that obtained by purification with two passes through cesium chloride gradients—the most rigorous method for DNA purification.

• Purify high-quality DNA in Less than two hours for transfections and other applications with no need for additional steps to remove contaminants like RNA, proteins, or endotoxins
• Additionally, elimination of phenol, chloroform, ethidium bromide, and cesium chloride from protocol minimizes exposure to and disposal of hazardous materials

PureLink HiPure Plasmid Filter Kits provide:

• Simple protocols—bacterial lysate clarification without centrifugation
• Fast results save time by purifying plasmid DNA in less than two hours
• High yields up to 200 μg for midipreps and up to 850 μg for maxipreps
• Reliable performance, high-quality plasmid DNA purified with these kits is suitable for wide variety of applications

How it works

• Addition of a HiPure Filter Column to the PureLink HiPure Plasmid Kits provides rapid clearing of the bacterial lysate without the need for centrifugation
• Lysate filtration cartridge is integrated into DNA-binding column for a combined step of lysate clarification and plasmid DNA binding directly to the anion-exchange resin
• Single column wash removes impurities such as RNA, proteins, metabolites, and other low molecular weight molecules; the ultrapure plasmid DNA is then eluted with high-salt buffer
• DNA is then desalted and concentrated with an isopropanol precipitation step, then collected by centrifugation