It is used as a pH indicator and to measure serum albumin concentrations. It is used as a pH indicator (5.2-6.8 : yellow to blue-purple) and also used in spectrophotometric determination of serum-albumin.
This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.
Applications
It is used as a pH indicator and to measure serum albumin concentrations. It is used as a pH indicator (5.2-6.8 : yellow to blue-purple) and also used in spectrophotometric determination of serum-albumin.
Solubility
Insoluble in water. Soluble in ethanol (80 mg/ml), alcohol, methanol (4 mg/ml), methyl cellosolve (300 mg/ml) and dilute alkalies.
Notes
Transition interval: pH 5.2 (yellow) to pH 6.8 (purple)
RUO – Research Use Only
General References:
- Hill PG, et al. Bromocresol purple and the measurement of albumin. Falsely high plasma albumin concentrations eliminated by increased reagent ionic strength.Ann Clin Biochem.1983,20(5), 264-70.
- Ueno T.; Hirayama S.; Sugihara M.; Miida T. ANNALS EXPRESS: The bromocresol green assay, but not the modified bromocresol purple assay, overestimates the serum albumin concentration in nephrotic syndrome through reaction with α2-macroglobulin.Ann. Clin. Biochem.2015.