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HisTrap™ HP Prepacked Columns
Purify histidine-tagged proteins directly from homogenized, unclarified cells. Cytiva HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).
| Catalog Number | Quantity | Diameter (Metric) Inner |
|---|---|---|
| FKS11773209 | 5 x 5 mL | 16 mm |
Catalog number FKS11773209
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Quantity:
5 x 5 mL
Diameter (Metric) Inner:
16 mm
- Prepacked with Ni Sepharose™ High Performance medium
- Negligible nickel ion leakage
- Pressure Max: 3 bar
- Column material: polypropylene
- 1 and 5mL columns
- Compatible with a wide range of reducing agents, detergents, denaturants, and other additives
- Manual operation with syringe, pump or chromatography system
Media
- Matrix: 6% cross-linked agarose
- Average particle size: 34μm
- pH stability working range: 3 to 12
1mL columns
- Bed Dimensions: 7 x 25mm
- Flow velocity: 4mL/min.
5mL columns
- Bed Dimensions: 16 x 25mm
- Flow velocity: 5mL/min.
Specifications
Bed Volume5 mL
Diameter (English) Inner0.6 in.
Diameter (Metric) Inner16 mm
DescriptionHisTrap HP are nickel-charged IMAC columns for high resolution his-tagged protein purification with high binding capacity for maximized recovery.
Flow Rate4 mL/min. (1 mL), 5 mL/min. (5 mL)
MaterialPolypropylene
pH Range3 to 12
Support Type6% Highly Cross-linked Agarose
Max. Operating Pressure5 bar (0.5 MPa, 70 psi)
Quantity5 x 5 mL
TypePre-packed Column
Unit SizePK
Contents & Storage
4°C to 30°C