For separation of cells, subcellular particles, and larger viruses (down to approx. 70S) under gentle conditions that preserve viability and morphological integrity. Cytiva™ Percoll™ Centrifugation Media Percoll is composed of colloidal silica coated with polyvinylpyrrolidone (PVP).
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Catalog Number
Quantity
FKS10607095
1 L
Catalog number FKS10607095
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Quantity:
1 L
Gradients can either be preformed or spontaneously generated by centrifugation at moderate speeds in an angle-head rotor
Low viscosity of media allows cell isolations on preformed gradients to be completed in only a few minutes using low centrifugal forces (200 to 1000xG)
Iso-osmotic gradients throughout cover a range of densities up to 1.3g/mL
Nontoxic, almost chemically inert and does not adhere to membranes
Adjustable to physiological ionic strength and pH; can be buffered within pH range 5.5 to 10.0 without change in properties
Percoll is a silica sol with nondialyzable PVP coating; 15-30nm dia. bead size